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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Neuropeptide Y Promotes Human M2 Macrophage Polarization and Enhances p62/SQSTM1-Dependent Autophagy and NRF2 Activation
doi: 10.3390/ijms232113009
Figure Lengend Snippet: Flow cytometric analysis of the M1 markers HLA-DR and CD16 and the M2 markers CD206 and CD163 on ( a ) M0, ( b ) polarized M(IFN-γ/LPS), and ( c ) polarized M(IL-10) macrophages. Macrophages (7 × 10 5 cells per mL) were stimulated or not with 10 −8 M NPY in the presence or absence of LPS in complete medium for 24 h and then analyzed for surface marker expressions by flow cytometry. Histograms show the percentages of positive cells (%) and the mean fluorescence intensity (MFI). Results are expressed as mean value ± SD of 4 independent experiments. Significance was determined by one-way ANOVA followed by Tukey’s post hoc analysis; *: p < 0.05, **: p < 0.01.
Article Snippet: To determine macrophage phenotypic surface markers, macrophages were stained with the following monoclonal antibodies (mAbs): phycoerythrin (PE)-CD163, fluorescein isothiocyanate (FITC)-CD206 and
Techniques: Marker, Flow Cytometry, Fluorescence